使える単語・表現集
4. 「分かるために何をしたか（ドン！）」で使える単語・表現
To elucidate the role of VirD5 in Agrobacterium infection, we examined possible interactions between VirD5 and other known exported Vir proteins in plants using bimolecular fluorescence complementation (BiFC)
To test this hypothesis, we first examined whether coexpression of VirF and VirD5 in N. benthamiana alters the cellular amounts of VirF.
Then, we analyzed the stability of VirF in the presence or absence of VirD5 by a cell-free degradation assay.
To shed light on the mechanism underlying the VirF degradation, we first tested the effect of the proteasome inhibitors, MG132 and lactacystin, on the stability of VirF.
To explore this possibility, we generated a plant dominant-negative CULLIN1
To better understand the plant-pathogen molecular arms race revolving around the SCF complex, we summarize recent advances in the studies of phytopathogen-encoded F-box effectors.
These results prompted us to investigate whether VirF undergoes ubiquitination in vivo.
For this purpose, Myc-VirF and FLAG-tagged ubiquitin (FLAG-Ub) were coexpressed in N. benthamiana leaves.
We took advantage of this molecular tool to test potential involvement of a host SCF complex in VirF degradation.
To circumvent this problem, it may be beneficial to utilize simpler model organisms, such as yeast, and directly analyze the behavior of T-DNA and the host factors at DSBs.
In this review, we summarize the recent advances in our understanding of Agrobacterium T-DNA integration.
In this review, we focus on the potential role of the DSB repair machinery in Agrobacterium genetic transformation.
In this paper, the authors devised an elegant mutant screen using the luciferase reporter fused to the promoter of HSP70.
In this paper, the authors utilized various in vitro assays, such as glycan array analysis and isothermal titration calorimetry.
In this paper, the authors conducted a yeast two-hybrid screen using the SUMO-conjugating enzyme (SCE) and the SUMO-specific protease (ESD4) as bait.
In this paper, the authors performed genome-wide transcript profiling.